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AJBLS · Lens · Nitrogen fixation by the Native Azospirillum spp. Ssolated from Rhizosphere and Non-Rhizosphere of foxtail Millet

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Article · Volume 1, Issue 3 · pp. 213–218

Nitrogen fixation by the Native Azospirillum spp. Ssolated from Rhizosphere and Non-Rhizosphere of foxtail Millet

Mokula Md. Raffi1,2, P.B.B.N. Charyulu1

  1. 1Department of Microbiology, Sri Krishnadevaraya University, Anantapur - 515003, INDIA.
  2. 2Department of Biotechnology, Sri Krishnadevaraya University, Anantapur-515003, INDIA.

Abstract

Experiments were conducted to isolate Azospirillum spp. from the rhizosphere and non-rhizosphere soil of foxtail millet grown in native black loamy soils of Anantapur district. A total of 100 strains of Azospirillum were isolated and grown on semi-solid nitrogen-free bromothymol blue malate semisolid malate agar (Nfb), purified and tested for nitrogen fixing abilities, only 56 strains of Azospirillum spp. strains showed nitrogen fixing ability, -1 out of which 20 strains fixed nitrogen efficiently. Azospirillum strains varied in their nitrogen-fixing abilities ranging from 2.95 to 12.4 mg g malate. All the strains were identified as Azospirillum lipoferum. These strains when tested for IAAproduction, all produced IAA.

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