A Study on Callus Induction and the Successful Regeneration of Plantlets from Rhizome Explants of Alpinia galanga (L.) Willd., a Valuable Medicinal Plant

Abstract

Aim: The primary focus of our study is to establish a reliable approach for plant regeneration via indirect organogenesis ofAlpinia galanga, utilizing rhizome buds.Materials and Methods:The rhizome explants were placed on MS media supplemented with 0.5 to 2.5 mg/L 2,4-D to induce callus formation. The more effective callus induction was observed on MS media containing 1.5 mg/L 2,4-D with a light green, compact nature of callus. The callus transferred to MS media supplemented with various cytokinin’s such as BAP, Kinetin and TDZ in different concentrations for shoot induction.Results:The highest shoot induction frequency (91.0±3.21%), the maximum number of shoots per callus (9.66±0.88) and the highest plantlet length (91.13±1.44 mm) were observed in 1.0 mg/L BAP. The best-regenerated shoot buds were then transferred to auxins such as NAA and IBA with various concentrations (0.25 mg/L, 0.5 mg/L, 0.75 mg/L and 1.0 mg/L) for root initiation. High rooting frequency (93.00±1.73%), maximum number of roots (21.66±1.76) and maximum root length (93.33±1.76 mm) were induced in MS medium containing 0.5 mg/L IBA.Conclusion:We have developed a reliable and easily replicable protocol for callus induction and subsequent plant regeneration ofA. galangain controlled laboratory settings. Following this, thein vitroplantlets were successfully acclimatized to field conditions.

Keywords: Alpinia galanga; Auxin; Callus; Rhizome