Evaluation of in vitro Anti-Inflammatory Activity of Leaves of Terminalia chebula Retz. (Combretaceae)

Abstract

Aim: The present study focused to establish thein vitroanti-inflammatory activity of leaf extracts ofTerminalia chebulaprepared using different solvents.Materials and Methods:Thein vitroantiinflammatory potentiality of leaves ofTerminalia chebulawere tested using the protein denaturation inhibition assay (bovine serum albumin inhibition assay).Results:Leaves ofTerminalia chebulawere extracted by ultrasonic assisted extraction using different solvents in increasing order of polarity. The extracts so obtained were screened for anti-inflammatory potential. Leaf extracts of different concentrations ranging from 6.25-100 μg/mL were incubated with bovine serum albumin and its ability to inhibit protein denaturation was measured. The percentage of inhibition of standard diclofenac sodium was determined and its IC50 value was found to be 49.57 μg/ mL. The ethanol, ethanol: distilled water extracts (4:1, 3:2, 2:3, 1:4) and distilled water extracts exhibited greater inhibition of protein denaturation and their IC50 values ranged from 52.03 μg/mL to 77.75 μg/mL.Conclusion:The current study proved that leaves ofTerminalia chebulaexhibited considerable amount of anti-inflammatory activity and could be used as anti-inflammatory drug but only after proper scientific validation (furtherin vitroandin vivostudies).

Keywords: Anti-inflammatory study; in vitro study; Protein denaturation assay; Terminalia chebula