Antibacterial Activity of Green Synthesized Copper Oxide Nanoparticles Using Muntingia calabura Leaf Extracts against Staphylococcus aureus and Escherichia coli

Background:The surge in bacteremia and healthcare-associated infections, driven by aging populations and antimicrobial resistance, prompted research into nanotechnology. Copper Oxide Nanoparticles (CuONPs) have emerged as a promising candidate due to their potent antibacterial properties. Muntingia calabura, renowned for its medicinal uses, presents a novel avenue for CuONP synthesis. This study aimed to evaluate the antibacterial efficacy of CuONPs against Staphylococcus aureusand Escherichia coli. Materials and Methods:M. calaburaleaves extracts were utilized to synthesize CuONPs from Copper (II) Sulfate Pentahydrate. CuONPs morphology was characterized by Scanning Electron Microscopy. Subsequently, the antibacterial properties of the CuONPs with various concentrations of 25%, 50%, 75% and 100% were analyzed against S. aureusand E. coliusing agar well diffusion and MIC determination vis-à-visgentamicin as the control. Results:The results showed antibacterial potential with 75% leaf extract concentration showing the highest Zone of Inhibition (ZOI) and 50% as Minimum Inhibitory Concentration (MIC) for S. aureus. While, 50% of the concentration showed the highest ZOI and MIC at 25% for E. coli. ANOVA and Tukey’s post-hocdetermined a significant difference between the different concentrations of the CuONPs (p-values=0.000 and 0.035). However, only the antibacterial activity of the CuONPs against E. colishowed no significant difference with the positive control (p-value=0.125). Conclusion:Although the synthesized CuONPs are deficient compared to positive control gentamicin, it manifests significant antibacterial potential against S. aureusand E. coliand is recognizable as a potential alternative in combating antibacterial resistance.