Article

Development of an IoT-Enabled Hybrid Bioreactor from Kitchen Waste and Bagasse for Decentralised Green Hydrogen Production

1, 1★★ Corresponding author

  1. 1Department of Bioinformatics, School of Life and Basic Sciences, Jaipur National University, Jaipur, Rajasthan, INDIA.

CORRESPONDENCE

Preenon Bagchi

Department of Bioinformatics, School of Life and Basic Sciences, Jaipur National University, Jaipur, Rajasthan, INDIA.

prithish.bagchi@gmail.com

Received: 21-04-2026; Revised: 07-05-2026; Accepted: 13-07-2026.

Volume 15, Issue 2 · pp. 481–487 · 2026 · DOI: 10.5530/ajbls.20260173

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ABSTRACT

Background: Fossil fuel dependency and the growing burden of organic waste disposal present urgent challenges, particularly in developing nations where decentralised energy solutions are most needed. Objectives: This study describes the concept and preliminary proof-of-concept development of a low-cost, IoT-enabled hybrid bioreactor intended to convert kitchen waste and sugarcane bagasse into green hydrogen through anaerobic dark fermentation and thermochemical gasification. Materials and Methods: The work began with the construction of a glass model reactor, which allowed direct visual observation of fermentation activity. Kitchen waste and bagasse were mixed into a slurry and subjected to anaerobic conditions; maintaining stable temperature and pH proved difficult without automated controls, but visible gas bubble formation was observed between 16 and 36 hours after loading. Results: A flame test on the collected gas produced a pale blue flame, providing initial qualitative confirmation of hydrogen generation. Conclusion: Based on these prototype observations and relevant literature, a stainless-steel reactor design (Grade 316L, 10-litre working capacity) was proposed, incorporating a sensor suite -including DS18B20 temperature probes, industrial pH electrodes, piezo-resistive pressure transducers, and MQ-8 hydrogen sensors -coordinated by an ESP32 microcontroller with cloud-based data transmission via Thing Speak. Projected operational estimates for co-processing approximately 2 kg kitchen waste and 1.5 kg bagasse over a 48-hr cycle suggest a hydrogen production rate of around 0.7 L/hour, yielding roughly 19.2 L per kilogram of total waste input under stable mesophilic conditions (pH 6.8-7.2; 35-45ºC). The modular system architecture is designed for scalable deployment from households to community level. This article presents the prototype findings, proposed system design, and estimated performance data alongside a review of the relevant literature.

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    Meena, S., & Bagchi, P. (2026). Development of an IoT-Enabled Hybrid Bioreactor from Kitchen Waste and Bagasse for Decentralised Green Hydrogen Production. Asian Journal of Biological and Life sciences, 15(2), 481–487. https://doi.org/10.5530/ajbls.20260173