Article · Volume 2, Issue 1 · pp. 50–53
The Effects of Cryptelytrops albolabris, Calloselasma rhodostoma and Daboia siamensis Venoms on Human Cancer Cells
Suchitra Khunsap1, Supranee Buranapraditkun2, Sunutcha Suntrarachun1, Songchan Puthong3, Orawan Khow1, Pannipa Chulasugandha1, Supatsorn Boonchang1
- 1Research of Development, Queen Saovabha Memorial Institute, Bangkok 10330, THAILAND.
- 2Cellular Immunology Laboratory Allergy and Clinical Immunology Unit, Department of Medicine, Faculty of Medicine Chulalongkorn, University, Bangkok 10330, THAILAND.
- 3Institute of Biotechnology and Genetic Engineering, Chulalongkorn University Institute Building 3, Phayathai Road, Patumwan, Bangkok 10330, THAILAND.
Abstract
Three snake venoms: Cryptelytrops albolabris, Calloselasma rhodostoma and Daboia siamensis were studied for cytotoxic activity and apoptosis on five cancer cell lines; BT474, SW620, KATO-III, Hep-G and ChaGo. Cytotoxic effect was determined by MTT assay and flow cytometry has 2 been used for apoptosis. Various concentrations of three venoms showed cytotoxic against cancer cells as time-dependent. The potent of cytotoxic from C. albolabris venom against cancer cells were: BT474 (2.96 ± 0.44 µg/ml), SW620 (3.32 ± 0.14 µg/ml), KATO-III (3.72 ± 0.11 µg/ml) and Hep-G (3.74 ± 0.43 µg/ml), respectively. C. rhodostoma venom showed cytotoxic to BT474 (3.16 ± 0.69 µg/ml), SW620 (3.5 ± 0.01 µg/ml) and KATO¬ 2 III (3.74 ± 0.37 µg/ml) while the venom of D. siamensis was highly toxic to only ChaGo (0.48 ± 0.04 µg/ml). Apoptotic cell death using PI staining was dose and time dependent.
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