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FRA · Lens · Comparative Study of Antioxidant Activities of Selected Medicinal Plants of Shujabad Area in Multan, Pakistan

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Article · Volume 11, Issue 2 · pp. 46–51

Comparative Study of Antioxidant Activities of Selected Medicinal Plants of Shujabad Area in Multan, Pakistan

Salma Ghaffar1, Shazia Jabeen2, Tahir Mehmood3, Muhammad Qasim Hayat4, Mudassir Iqbal5

  1. 1Department of Botany, The Women University Multan, Multan, PAKISTAN.
  2. 2Department of Environmental Sciences, The Women University Multan, Multan, PAKISTAN.
  3. 3Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences-VVAS, Lahore, PAKISTAN.
  4. 4Medicinal Plants Research Laboratory, Department of Plant Biotechnology, Atta Ur Rehman School of Applied Biosciences (ASAB), National University of Sciences and Technology (NUST), Islamabad, PAKISTAN.
  5. 5Department of Chemistry, School of Natural Sciences, National University of Sciences and Technology (NUST), Islamabad, PAKISTAN.

Abstract

Background: In humans, especially in elderly patients, free radicals and oxidative stress are one of the main reasons behind a number of diseases/disorders, such as cardiovascular, pulmonary and neuronal in nature. Therefore, it is imperative to investigate substances/compounds that possess potent free radical scavenging activity, especially, from the indigenous flora.Methods: The present study was aimed to screen the free radical scavenging activity of ethanolic and methanolic leaf extracts of three medicinal plants: Syzygium cumini, Psidium guajava and Callicarpa dichotoma. The antioxidant activity was determined using 1,1-Diphenyl-2-picryl hydroxyl (DPPH) and Hydrogen peroxide (H2O2) scavenging assays. Various concentrations (250, 500, 750 and 1000µg/ml) of plant extract were used to carry out the assays and Ascorbic acid was used as the standard. The free radical quenching potential was expressed in inhibition percentage (%) and concentrations were expressed in µg/ml. Optical density of DPPH and H2O2 was measured using spectrophotometer at 517 and 230 nm, respectively.Results: Results from the DPPH and H2O2 assays showed that antioxidant activities were observed to be highest in P. guajava (89 and 81% respectively) followed by S. cumini (84 and 72% respectively) and C. dichotoma (83 and 70% respectively) in the ethanolic extracts.Conclusion: It is concluded that the selected plant materials used for the study have a powerful antioxidant potential and could be used in various therapeutic and medicinal applications.

Keywords: ROS; Antioxidant; C. dichotoma; P. guajava; S. cumini; DPPH assay; H2O2 assay

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