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FRA · Lens · Antioxidant activity profiling by spectrophotometric methods of phenolic extract of Prasium majus L

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Communication · Volume 3, Issue 1 · pp. 43–46

Antioxidant activity profiling by spectrophotometric methods of phenolic extract of Prasium majus L

Tarik Md. Chaouche1, Farah Haddouchi1, Riadh Ksouri2, Faten Medini2, Imad A. El-Haci1, Zahia Boucherit3, Fatima Zohra Sekkal4, Fawzia Atik-Bekara1

  1. 1Laboratory of Natural Products, Department of Biology, Faculty of Sciences, Tlemcen University, Tlemcen, Algeria.
  2. 2Laboratory of Extremophile Plants, Borj Cedria Biotechnology Centre (CBBC), Hammam-Lif 2050, Tunisia.
  3. 3Antibiotics Antifungal Laboratory: Physical-Chemistry, Synthesis and Biological Activity, Department of Biology, Faculty of Sciences, Tlemcen University, Tlemcen, Algeria.
  4. 4Department of Biotechnology, Faculty of Natural Science and Life, Abdelel Hamid Ibn Badiss University, Mostaganem 27000, Algeria.

Abstract

Introduction: Phytochemicals are extensively found at different levels in many medicinal plants. To investigate the phenolic compound content and in vitro antioxidant activity of phenolic extract from Prasium majus L (Lamiaceae).Methods: The present investigation comprises, estimation of total polyphenol, flavonoid, tannin, in vitro antioxidant assays such as total antioxidant capacity, DPPH, ABTS, b-carotene and ferric reducing power.Results: P. majus exhibited 64.25 mg GAE g�1 extract of polyphenol phenol content, and better scavenging activity of DPPH (IC50 ¼ 7.95 mg mL�1), ABTS�þ (IC50 ¼ 373.78 mg mL�1), and b-carotene (IC50 ¼ 122.56 mg mL�1).Conclusion: Our results clearly demonstrated that phenolic extract P. majus has antioxidant capacity. Therefore is a valuable source of natural antioxidants.

Keywords: Prasium majus; Quantification of phenolic classes; Antioxidant activity

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