Antioxidant Activities of Root, Stem and Leaves of Vernonia ci nerea (L) Less.

Abstract

Background: Vernonia cinerea (L) Less has ethnomedicinal importance and is well known for its anti-inflammatory and anticancerous properties.Objective: Present study investigates antioxidant activity of various crude extracts prepared from different organs of plant such as Root, Stem and Leaves using polar and nonpolar solvents.Materials and methods: The antioxidant activity was determined using different methods which includes radical scavenging assays (DPPH and ABTS) and phosphomolybdenum assay. LC- MS profiling was performed for the extracts showing maximum antioxidant activity to identify bioactive constituents.Result: Difference was observed in the antioxidant activities of extracts by different methods. Significant (p< 0.05) antioxidant activity was exhibited by different organs of a plant and different extracts. LC-MS analysis revealed the presence of phenols, terpenoids, quinones and steroids in the extracts.Conclusion: Different antioxidant assays help in knowing the antioxidant potential of different crude extracts. The bioactive constituents identified can be correlated to antioxidant activity and used in drug formulations.

Keywords: ABTS; DPPH; LC-MS; Phosphomolybdenum; Vernonia cinerea