Article · Volume 8, Issue 2 · pp. 70–73

Preparation, Characterization and Electron Paramagnetic Resonance (EPR) Spectroscopic Studies of OXANOH

Nahidh W Hasaniya^1,4, Shyamal Premaratne^2,4, Wayne W Zhang^3,4, Aziz M Razzuk⁴, J Judson McNamara⁴, Roderick H Dashwood⁵

¹Department of Cardiovascular Surgery, Kaiser Permanente Medical Group, Fontana, California, USA..
²Hunter Holmes McGuire Veterans Ad ministration Medical Centre, Richmond, Virginia, USA..
³Department of Surgery, University of Washington and VA Puget Sounds Healthcare System, Seattle,Washington, USA..
⁴Department of Surgery, John A. Burns School of Medicine, University of Hawaii and Research Laboratory at the Queen’s Medical Centre, Honolulu, Hawaii, USA..
⁵Director, Center for Epigenetics and Disease Prevention, Texas A and M College of Medicine, Houston, Texas, USA..

Abstract

Objective: Hydrogenation of the stable free radical, 2-Ethyl-2,5,5-trimethyl-3-oxazolidinoxyl (OXANO•), converts it to the intracellular spin trap, 2-Ethyl-1-hydroxy-2,5,5-trimethyl-3-oxazolidine (OXANOH).Methods: Due to the flammable nature of the reaction, this procedure must be carried out with extreme care. OXANO• (10 mM) was diluted in 50 ml of Krebs-Henseleit buffer (pH 7.4) and hydrogenated in a nitrogen atmosphere in the presence of Platinum (IV) Oxide Hydrate (100 mg).Results: The reaction was completed in approximately 45 min, and was confirmed by the disappearance of the three-electron paramagnetic resonance (EPR) signals (1:1:1). Additionally, the absorption spectra were measured for both OXANOH and OXANO• in the ultraviolet (UV) and visible light regions.Conclusion: OXANOH is an extremely valuable oxygen free radical spin trap due to its ability to trap both extra and intracellular free radicals in biological tissues.

Keywords: Ischemia-reperfusion injury; Reactive oxygen species; Perfusion; Lung damage; Scanning electron microscopy

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