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IJPI · Lens · Synthesis, Characterization, RP-HPLC Method Development and Validation for Qualitative Estimation of (4Z) 3 Methyl 1 (4 Nitrobenzoyl) 1H Pyrazole 4,5 Dione 4[(4fluorophenyl) Hydrazone]

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Article · Volume 13, Issue 2 · pp. 313–320

Synthesis, Characterization, RP-HPLC Method Development and Validation for Qualitative Estimation of (4Z) 3 Methyl 1 (4 Nitrobenzoyl) 1H Pyrazole 4,5 Dione 4[(4fluorophenyl) Hydrazone]

Harsha Ashtekar1, Natasha Aggarwal2, Chaitra Raviraj3, Gupta Dheeraj Rajesh4, Nimmy Varghese1

  1. 1Department of Pharmacology, NITTE (Deemed to be University), NGSM Institute of Pharmaceutical Sciences (NGSMIPS), Mangalore, Karnataka, INDIA.
  2. 2Department of Pharmaceutical Chemistry, Yenepoya University (Deemed to be University), Yenepoya Pharmacy College and Research Center, Mangalore, Karnataka, INDIA.
  3. 3Department of Advance Research Centre, (NGSMIPS), NITTE (Deemed to be University), NGSM Institute of Pharmaceutical Sciences Mangalore, Karnataka, INDIA.
  4. 4Department of Pharmaceutical Chemistry, NITTE (Deemed to be University), NGSM Institute of Pharmaceutical Sciences (NGSMIPS), Mangalore, Karnataka, INDIA.

Abstract

Aim: A new simple, accurate, precise, Reverse Phase High-performance Liquid Chromatography method was developed and validated for the estimation of newly synthesized (4Z) 3 methyl 1 (4 nitrobenzoyl) 1H pyrazole 4,5 dione 4[(4fluorophenyl)hydrazone] derivative of pyrazolone.Materials and Methods: The chromatogram was run through Luna 5µ C18 (2). 250 × 4.80 mm. 272817-7 HPLC column; mobile phase consisting of acetonitrile and water in the ratio 90:10 was pumped through the column at a flow rate of 0.8mL/min.Results: The optimized wavelength was 237 nm. The retention time of (4Z) 3 methyl 1 (4 nitrobenzoyl) 1H pyrazole 4,5 dione 4[(4fluorophenyl)hydrazone] was found to be 7.3 min. The percentage relative standard deviation was obtained as 0.3%.Conclusion: This method was accurate, precise, and sensitive; therefore, could be used for the quality control and bioanalytical evaluation of (4Z) 3 methyl 1 (4 nitrobenzoyl) 1H pyrazole 4,5 dione 4[(4fluorophenyl)hydrazone] in drug testing laboratory.

Keywords: Column Chromatography; HPLC; Pyrazolone derivative

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