Article · Volume 1, Issue 6 · pp. 421–427

Molluscicidal activity against Oncomelania hupensis of endophyte JJ18 from Pseudolarix kaempferi Gord

Chen Jun¹, Han Bang-xing^1,2, Guo Shang-bin¹, Wang Yan¹, He Jia², Zhou Xiao-kun¹, Yang Xun¹, Han Fang-an⁴

¹College of Pharmacy, Jiangsu University, Zhenjiang, Jiangsu, P. R. China
²Research Center of Plant Cell Engineering, West Anhui University, Lu’an, Anhui, P. R. China
³School of Food & Bioengineering, Henan University of Science and Technology, Luoyang, P. R. China
⁴Zhenjiang Institute of Parasitic Diseases, Zhenjiang, Jiangsu, P. R. China

Abstract

To screen a kind of biological controlling measure against the Oncomelania hupensis snails, This study is to research the molluscicidal effect of JJ18 and identify its species. The alcoholic extract of JJ18 broth effectively killed O. hupensis snails, and the n-butanol fraction of the extract had the best molluscicidal activity compared with other fractions. Ochratoxin A was detected from JJ18 broth with ELISA analysis, and it may be a molluscicidal active substance in the broth. JJ18 strain belongs to Aspergillus niger was identified by morphology characteristics of the slide culture and rDNA ITS sequence analyzing.

Keywords: Oncomelania hupensis; Endophyte; molluscicidal activity; Pseudolarix kaempferi Gord; ITS sequence

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