Article · Volume 10, Issue 4 · pp. 404–407

Seabuckthorn (Hippophae rhamnoides and Hippophae salicifolia) Seed Oil in Combating Inflammation: A Mechanistic Approach

Suchita Dubey¹, M. V. Ramana¹, Anuradha Mishra², Pushpraj S. Gupta³, Himani Awasthi¹

¹Department of Pharmacology, Amity Institute of Pharmacy, Amity University, Lucknow Campus, INDIA.
²Department of Pharmacology, Faculty of Pharmacy, Integral University, Lucknow, INDIA.
³Department of Pharmacy, Faculty of Health and Allied Sciences, SHIATS, Formerly Allahabad Agricultural University, Allahabad, Uttar Pradesh, INDIA.

Abstract

Objective: This study assessed and comparedin vivoanti‑inflammatory activity of Hippophae rhamnoides (HR) andHippophae salicifolia (HS) seed oil.Materials and Methods: HR and HS seed oil was extracted by Soxhlet apparatus and characterized using gas chromatography mass spectroscopy. Wistar rats were used for predicting anti‑inflammatory activity.Results: HR and HS (2 and 4 ml/kg, respectively) exhibited dose‑dependent inhibition of carrageenan‑, histamine‑, prostaglandin‑, bradykinin‑, and arachidonic acid (AA)‑induced paw edema. Significant leukotriene‑induced inhibition was observed in HR. Myeloperoxidase (MPO) and lipid‑peroxidase (LPO) assays were performed and HR and HS seed oil significantly decreased the level of MPO and LPO at 4 ml/kg dose (P< 0.001).Conclusion: Dual inhibition of AA metabolism in HR and cyclooxygenase inhibition in HS was observed that might be attributed to the presence of polyunsaturated fatty acids (PUFAs), specifically, a correct balance of n‑3 and n‑6 PUFAs. However, the findings should be interpreted in the light of limitation of this study. Detailed experimentation at enzymatic levels would further help in substantiating the results inferred in this study.

Keywords: Antibradykinin; Antihistaminic; Arachidonic acid; Leukotriene; Polyunsaturated fatty acid; Prostaglandin

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