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PR · Lens · Cytotoxic and Antimigratory Effects on Michigan Cancer Foundation‑7 Cells of Morinda citrifolia L. Leaf Extract and Formulation of Tablets from Extract

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Article · Volume 12, Issue 1 · pp. 24–28

Cytotoxic and Antimigratory Effects on Michigan Cancer Foundation‑7 Cells of Morinda citrifolia L. Leaf Extract and Formulation of Tablets from Extract

Supavadee Boontha1, Benjaporn Buranrat2, Tasana Pitaksuteepong3

  1. 1Department of Pharmaceutical Sciences, School of Pharmaceutical Sciences, University of Phayao, Phayao, THAILAND.
  2. 2Faculty of Medicine, Mahasarakham University, Maha Sarakham, THAILAND.
  3. 3Department of Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, Naresuan University, Phitsanulok, THAILAND.

Abstract

Background: Cancer is one of the deadliest diseases known to man. Efforts to combat the disease remain ongoing. Morinda citrifolia L. is a medicinal plant which is gaining interest as a natural chemotherapeutic agent for breast cancer treatment.Objective: This study aimed to determine the anticancer activity of M. citrifolia leaf extract on Michigan cancer foundation 7 (MCF‑7) breast cancer cells as well as formulate tablets containing the extract to assess their viability.Materials and Methods: M. citrifolia leaf extract was prepared using the maceration method. Cytotoxicity and cell migration suppression, representing anticancer activity, were also assessed in MCF‑7 cells by the sulforhodamine B assay and wound‑healing assay. Tablets containing the extract were formulated using the wet granulation method. The prepared tablets were then evaluated regarding their physical properties and scopoletin content, a marker in the extract, before and after stability testing.Results: The extract showed cytotoxicity on MCF‑7 cells in a dose‑ and time‑dependent manner with 50% inhibitory concentration values of 39.9 ± 3.5 µg/mL for 48 h. In addition, it showed an antimigratory effect on MCF‑7 cells with a significant effect at 25 µg/mL. The prepared tablets had good characteristics and were found to meet the requirements of the United States Pharmacopeia. Moreover, they could maintain their physical properties and scopoletin content over a 2‑month period.Conclusion: This study showed that M. citrifolia leaf extract exhibited potential anti‑breast cancer activity. The prepared tablets containing the extract could be a potential formulation for breast cancer treatment. However, the underlying mechanism of the extract as an antibreast cancer agent requires further investigation in subsequent study.

Keywords: Antibreast cancer; antimigratory effect; Morinda citrifolia L. leaf; scopoletin content; tablets

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