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PR · Lens · Larvicidal Efficacy of Andrographis paniculata and Tinospora cordifolia against Aedes aegypti: A Dengue Vector

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Article · Volume 12, Issue 4 · pp. 352–360

Larvicidal Efficacy of Andrographis paniculata and Tinospora cordifolia against Aedes aegypti: A Dengue Vector

Anubrata Paul1, V Samuel Raj1, Arpana Vibhuti1, Ramendra Pati Pandey1

  1. 1Centre for Drug Design Discovery and Development (C4D), SRM University, Sonepat, Haryana, INDIA.

Abstract

Background: Aedes aegypti, dengue fever mosquito, is predominantly accompanying the transmission of dengue in tropical and subtropical countries of the world, causing millions of deaths every year.Materials and Methods: The current research was carried out to evaluate the larvicidal efficiency of Andrographis paniculata and Tinospora cordifolia against third instar larvae ofA. aegyptiusingin silico and experimental study.Results: The methanolic extract of A. paniculata and ethanolic extract of T. cordifoliashowed the highest larvicidal activity with LC50 and LC90 value of 15.75 ppm; 349.60 and 100.64 ppm; 386.37 ppm, respectively after 48 h exposure, which was statistically significant (P = 0.05). However, the combined action of methanolic extract of A. paniculata+ ethanolic (50:50) extract of T. cordifoliaand the bioactive fraction of methanolic extract of A. paniculata showed the effective larvicidal activity with LC50 value of 113.20 ppm and 236.08 ppm respectively after 24 h treatment which was statistically significant (P = 0.05). The m/z values in liquid chromatography-mass spectrometry graph of bioactive fraction of methanolic extract of A. paniculata predicted the possible amount of bioactive compound class like flavone, phenols, phenylpropanoids, flavonoids, phenolic acid, alkaloids, isopalmitic acid, diterpenoids and quinic acid. The best energy binding affinity score against sterol carrying protein-2 of A. aegypti,panicolin (-9.86 kcal/mol), berberine (-10.13 kcal/mol) and their combination (50:50) showed effective larvicidal movement with LC50 value of 25.41 ppm, 23.15 ppm and 17.56 ppm after 24 h treatment.Conclusion: The crude extracts, bioactive fractions and synthetic bioactive compounds and their combination study of A. paniculata andT. cordifolia can be utilized as bio-control agents againstA. aegyptimosquito.

Keywords: Aedes aegypti mosquito larvae; Andrographis paniculata; Chromatographic techniques; In silico study; Larvicidal activity; Phytochemical screening; Tinospora cordifolia

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