Article · Volume 13, Issue 4 · pp. 233–237
Phytochemical and GC-MS Analysis of Hydro Ethanolic Leaf Extract of Ocimum sanctum (L.)
Atul Srivastava1, Subhashini s2, Anand Kumar Keshari1, Ragini Srivastava1
- 1Department of Biochemistry, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, INDIA.
- 2Department of Zoology, MMV, Banaras Hindu University, Varanasi, Uttar Pradesh, INDIA.
Abstract
Background: The pharmacological efficiency of herbal drugs has shown to be effective as conventional pharmaceutical drugs. Preliminary screening of herbal extracts helps in analysing the bioactive compounds present. Ocimum sanctum, the queen of herbs keeps a spiritual importance in Indian culture and have important place in traditional medicinal system of India.Objectives: The present study was implement to investigate the preliminary phytochemical screening and GC-MS analysis of leaf extract of O. sanctumto determine the phytoconstituents.Materials and Methods:Leaves collected were dried, crushed and mixed with ethanol and water and further applied for extract preparation. The prepare extract was used for phytochemical and GC-MS analysis.Results: In qualitative phytochemical analysis tannins, carbohydrate, quinine, anthraquinones, coumarins, phlobatanins, alkaloids, flavonoids, glycosides, terpenoids, diterpenes, phenols were found present while GC-MS analysis showed Eugenol, Cyclohexane, bicyclo[7.2.0]undec-4-ene, 4,11,11-trimethyl-8-methylene, Oxatricyclo[ 8.2.0.0(4,6)]dodecane,,12-trimethyl-9-methylene, Tetracontane, Phytol were present in majority.Conclusion: The study concluded that O. sanctumleaf extracts contain many biological active compounds which could be exploited for a development of plant based drug.
Keywords: Flavonoids; GC-MS Analysis; Herbal drugs; Phenolics; Phytochemical
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