Detection of HPTLC Fingerprint in Aerial Parts of Aerva lanata Linn. HPTLC Finger Print Profile and Evaluation of in vitro Antioxidant Activity of Aerva lanata Linn. Juss

Abstract

Background: Natural products enhance the standard of living, which greatly contributes to maintaining human health. Associated oxidative stress-mediated problems have been treated using Aerva lanata Linn. Juss in traditional and folkloric medicine.Objectives: The research aims to analyze the phytochemical profile using HPTLC of MEAL (methanol extracts of Aerva lanata) and AEAL (aqueous extracts of Aerva lanata) and to determine in vitroantioxidant capacity of MEAL and AEAL. The use of HPTLC fingerprint analysis will aid in identifying and monitoring drug effectiveness and ensuring therapeutic efficacy.Materials and Methods: Total phenol content, free radical scavenging efficiency of DPPH (1,1 diphenyl 2 picryl hydrazyl), ferric ion reducing antioxidant, and phosphomolybdate assays were used to determine in vitroantioxidant activity at various concentrations.Results: The number of phyto-constituents in MEAL at 375 nm and 550 nm was found to be more than AEAL. MEAL had a, stronger DPPH free radical scavenging capacity, higher phenol content, lower ferric ion antioxidant potential, and lower phosphomolybdenum assay. The natural phytoconstituents present in the plant extracts are responsible for antioxidant activity. As sample concentration increases, the antioxidant activity becomes more effective.Conclusion: The outcome results of the present investigation suggested that aerial parts ofAerva lanata could provide a source of natural antioxidants.

Keywords: Aerva lanata; DPPH; Fingerprint profile; HPTLC; in vitro antioxidant activity; Physicochemical parameters