Phyto-Analytical Studies and in vitro Wound Healing Activity of Synedrella nodiflora (L.) Gaertn Leaf Extract

Abstract

Background Traditionally among the Nigerian population Synedrella nodiflora is prominently used for treating Putrefied Wound. Objectives The present work was designed to evaluate the In vitro Wound healing activity of Synedrella nodiflora leaf extracts. Materials and Methods The current research work was aimed to perform the Qualitative phytochemical screening, FTIR, ICP-OES, Quantitative estimation of total phenolic and flavonoid content and in vitro pharmacological assay includes Antioxidant assay, Protein denaturation assay, cell line scratch assay for wound healing of EAE and EEs of Synedrella nodiflora. Results Secondary phytochemicals like Flavonoid, Alkaloid, Tannin, Phenols, Terpenoids and Steroids were reported. The ICP-OES analysis concluded that the presence of elements like Zn, Fe, Cu, Mg and heavy metals like Ni, Pb, Cr and Cd were within their limits as per WHO. The EAE and EE of Synedrella nodiflora were assessed for their total phenolic and flavonoid content. The Antioxidant activity of EAE and EE showed IC50 value 2.24±0.02 mg/mL and 3.72±0.02 mg/mL compared with standard Ascorbic Acid of 42.76±0.01 µg/mL. The % Inhibition of standard Diclofenac shows IC50 value 27.99±0.026 µg/mL. whereas the IC50 values of EAE and EE were found to be 294.98±0.02 µg/mL and 138.98±0.05 µg/mL respectively. The relative cell migration of EAE and EE were 0.041±0.05 mm and 0.036±0.01 mm at 2mcg. Conclusion These studies concluded that the Synedrella nodiflora leaf extracts were rich in various phytochemicals and can be used for treating wounds due to its antioxidant, anti-inflammatory and wound healing potential.

Keywords: Synedrella nodiflora; Phytochemical screening; Flavonoid; Phenolics; Wound Healing