Evaluation of in vitro Anti-Inflammatory Activity of Trichosanthes palmata against the Denaturation of Protein

Background: Trichosanthes palmata Lour. (Cucurbitaceae) is one of the important medicinal plants used traditionally for the treatment of asthma, earache, ophthalmia, leprosy, fever as well as used in the treatment of migraine. To validate folk used of Trichosanthes palmata as anti-inflammatory remedy. The leaves of Trichosanthes palmata were used for successive extraction (Maceration) with increasing polarity solvents. Ethanolic extract was selected for anti-inflammatory activity. In vitro anti-inflammatory activity was evaluated using protein denaturation method at different concentrations. Diclofenac sodium was used as standard drug.

Objectives: To evaluate the in vitro anti-inflammatory effect of ethanolic extract of leaves of Trichosanthes palmata against the denaturation of protein.

Materials and Methods: Inhibition of protein denaturation method was evaluated for anti-inflammatory activity.

Results: The ethanolic extract of Trichosanthes palmata exhibited anti-inflammatory activity by protein denaturation method.

Conclusion: The ethanolic extract from Trichosanthes palmata leaves exhibited significantly enhanced anti-inflammatory activity with increasing concentrations. This effect is likely attributed to the presence of active phytocompounds, including flavonoids, triterpenoids and related polyphenols. Consequently, Trichosanthes palmata holds potential as an anti-inflammatory agent.