Article · Volume 5, Issue 2 · pp. 86–102

Regeneration of multiple shoots from petiole callus of Viola serpens Wall

Umashankar Radheshyam Vishwakarma¹, Arun Manohar Gurav¹, Prakash Chandra Sharma¹

¹Department of Botany, National Research Institute of Basic Ayurvedic Sciences, Kothrud, Pune, MH, India

Abstract

Experiments were conducted to develop methodology for in vitro propagation and rapid multiplication of Viola serpens Wall. using petiole explant. The MS medium supplemented with 2, 4-D (6.78 mm) was found most suitable for callus induction in petiole explant. The best growth response and higher rate of shoot regeneration from petiole callus was observed on MS medium containing BAP (11.10 mm) as the average number of shoots could be increased to 36.4 on fourth successive subculturing. Higher rooting response with larger number of roots were observed in shoots inoculated on the half-strength MS medium supplemented with IBA (19.68 mm).

Keywords: Callus; growth regulators; multiple shoots; petiole; Viola serpens

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