The effect of 30% ethanolic extract of Indian propolis on replica of human dentin compared against commercially available desensitizing agent: A methodological SEM study in vitro

Abstract

Objective This study evaluated the ability of 30% ethanolic extract of Indian propolis on dentinal tubule occlusion comparatively against CPP-ACP containing desensitizing agent GC tooth mousse. Methodology The specimens were prepared from 30 freshly extracted sound human third molars stored in 10% formalin (pH 7.0) at a room temperature. From each specimen, a sectioned sample (5 mm length × 5 mm width × 3.5 mm depth) was obtained including the cervical area. Samples were smoothened and wet-polished with 1000- and 1200-grit aluminum oxide abrasive paper and diamond pastes, in order to stimulate the clinical aspect of hypersensitive dentin cervical surfaces. All the specimens were randomly assigned to three groups (n = 10), according to dentin surface treatments. Negative control: Untreated specimens (n = 4) and pretreated with 6% citric acid (n = 6); Test Group: 30% ethanolic extract of Indian propolis (n = 10); Positive Group: GC Tooth Mousse (n =10). All the specimens were prepared for SEM analysis. Results GC tooth mousse promoted tubule occlusion by crystal-like deposits in the lumen of the tubules. While propolis created a thin, smooth layer over dentin surface. Conclusion According to the SEM analysis, both desensitizing agent were able to occlude the dentinal tubules.

Keywords: Dentin hypersensitivity; dentinal tubule occlusion; desensitizing agents; GC tooth mousse; propolis