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PR · Lens · Development and validation of a high performance thin layer chromatographic method for determination of 1, 8-Cineole in Callistemon Citrinus

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Article · Volume 6, Issue 2 · pp. 143–147

Development and validation of a high performance thin layer chromatographic method for determination of 1, 8-Cineole in Callistemon Citrinus

Archana Shaha1, Vijay R Salunkhe1

  1. 1Department of Quality Assurance, Rajarambapu College of Pharmacy, HOD Pharmacognosy, Rajarambapu College of Pharmacy, Kasegaon, Maharashtra, India

Abstract

A new, simple, precise, rapid, and selective high performance thin layer chromatographic (HPTLC) method has been developed and validated for the estimation of 1, 8-cineole in volatile oil of leaves of Callistemon citrinus obtained by hydro distillation. The method was validated as per ICH guidelines and can be utilized for routine analysis. The retention factor for 1, 8-cineole was found to be 0.52. The linearity was found to be in the range of 3 μg-12 μg. The recovery obtained for 1, 8-cineole was 98%, which is satisfactory. The result obtained in validation indicate the accuracy, reproducibility, and reliability of the developed HPTLC method for determination of 1, 8-cineole.

Keywords: 1; 8-cineole; Callistemon citrinus; essential oil; high performance thin layer chromatographic

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