Article · Volume 7, Issue 2 · pp. 193–197
A study of Nigella sativa induced growth inhibition of MCF and HepG2 cell lines: An anti-neoplastic study along with its mechanism of action
Y. Padmanabha Reddy1, K. B. Chandrasekhar3, Mohammed Jaffar Sadiq2
- 1Departments of Pharmaceutcal Analysis
- 2Pharmacology, Raghavendra Institute of Pharmaceutical Education and Research
- 3Department of Chemistry, JNTUA, Ananthapuramu, Andhra Pradesh, India
Abstract
Objective To evaluate the anticancer potential of seeds of Nigella sativa using MCF and HepG2 cell lines along with its mechanism of action. Materials and Methods (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay and acridine orange/ethidium bromide nuclear staining technique were selected to evaluate anticancer potential and mechanism of action of test extract. Results Aqueous extract of N.sativa at a test dose of 180 mg and 300 mg was identified to be the best as anticancer agent against MCF and HepG2 cell lines among different solvent test extract where doxorubicin and cisplatin were employed as standard references. Discussion Further study including separation and characterization of active principles in the aqueous extract shall prove beneficial.
Keywords: Anti-neoplastic; HepG2; human breast adenocarcinoma cells; Nigella sativa seeds; Nuclear staining
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