Article · Volume 8, Issue 4 · pp. 281–286
Elucidation of Flavonoids from Carissa congesta, Polyalthia longifolia, and Benincasa hispida Plant Extracts by Hyphenated Technique of Liquid Chromatography-mass Spectroscopy
Gaurav M. Doshi1,4, Vivek V. Nalawade1, Aaditi S. Mukadam2, Pratip K. Chaskar1, Sandeep P. Zine1, Rakesh R. Somani3, Hemant D. Une5
- 1Departments of Pharmacology and Pharmaceutical Chemistry
- 2Departments of Quality Assurance
- 3Pharmaceutical Chemistry, Vivekanand Education Society’s College of Pharmacy, Mumbai, Maharashtra
- 4Pacific Academy of Higher Education and Research University, Udaipur, Rajasthan
- 5Department of Pharmacology, Y. B. Chavan College of Pharmacy, Rouzabagh, Aurangabad, India
Abstract
Background Carissa congesta (CC), Polyalthia longifolia (PL), and Benincasa hispida (BH) are economically important plants. Objective Current research encompasses identification of quercetin and rutin and their analogues by liquid chromatography-mass spectroscopy (LC-MS) from the selected plant species. Materials and Methods Fresh roots, leaves, and seeds of CC, PL, and BH plants respectively were shade-dried followed by extraction and elucidation of rutin and quercetin by LC-MS. Results Structural elucidation of CC, PL, and BH extracts revealed the presence of flavonoids such as quercetin (m/z 301) and rutin (m/z 610) as the parent ions along with presence of close analogues such as quercetin-O-hexoside, Vicenin 2, quercetin-3-O-xyloside/arabinoside, and quercetin-3-O-glucoside were identified as fragments. Conclusions Thus, CC, PL, and BH extracts revealed the presence of flavonoids belonging to the class of flavonols such as rutin and quercetin.
Keywords: Benincasa hispida; Carissa congesta; Liquid chromatography; Mass spectroscopy; Polyalthia longifolia; Quercetin; Rutin
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