Article · Volume 8, Issue 5S · pp. S1–S4
Anti-Leishmania Activity of Osthole
Elaheh Kordzadeh Kermani1, Seyed Ebrahim Sajjadi1, Seyed Hossein Hejazi2, Reza Arjmand3, Sedigheh Saberi2, Abbas Ali Eskandarian2
- 1Department of Pharmacognosy, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan
- 2Department of Medical Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan
- 3Department of Medical Parasitology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
Abstract
Background Treatment of cutaneous leishmaniasis (CL) is occasionally highly resistant to pentavalent antimonials, the gold standard in pharmacotherapy of CL. Since there is no effective vaccine, the discovery of natural antileishmanial products as complementary therapeutic agents could be used to improve the current regimens. Objective In this study in~vitro and in~vivo antileishmanial activities of osthole, a natural coumarin known to possess antibacterial and parasiticidal activities are evaluated. Materials and Methods Leishmania major infected J774.A1 macrophages were treated with increasing concentrations of osthole. CL lesions of BALB/c mice were treated topically with 0.2% osthole. Results Osthole exhibited dose-dependent leishmanicidal activity against intracellular amastigotes with IC50 value of 14.95 mg/ml. Treatment of CL lesions in BALB/c mice with osthole significantly declined lesion progression compared to untreated mice (P < 0.05), however did not result in recovery. Conclusion Osthole demonstrated remarkable leishmanicidal activity in~ vitro. Higher concentrations of osthole may demonstrate the therapeutic property in~vivo.
Keywords: Cutaneous leishmaniasis; Leishmania major; Leishmanicidal; Osthole
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