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PR · Lens · Analysis of Soluble Proteins in Natural Cordyceps sinensis from Different Producing Areas by Sodium Dodecyl Sulfate- Polyacrylamide Gel Electrophoresis and Two-dimensional Electrophoresis

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Article · Volume 9, Issue 1 · pp. 34–38

Analysis of Soluble Proteins in Natural Cordyceps sinensis from Different Producing Areas by Sodium Dodecyl Sulfate- Polyacrylamide Gel Electrophoresis and Two-dimensional Electrophoresis

Chun-Hong Li1, Hua-Li Zuo2, Qian Zhang1, Feng-Qin Wang1, Huan-Jia Hu2, Zheng-Ming Qian3, Wen-Jia Li3, Zhi-Ning Xia1, Feng-Qing Yang1

  1. 1Department of Pharmaceutical Engineering, School of Chemistry and Chemical Engineering, Chongqing University, Chongqing 401331, CHINA.
  2. 2State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao SAR, CHINA.
  3. 3Sunshine lake Pharma Co., Ltd., Guangdong 523850, CHINA.

Abstract

Background: As one of the bioactive components in Cordyceps sinensis (CS), proteins were rarely used as index components to study the correlation between the protein components and producing areas of natural CS.Objective: Protein components of 26 natural CS samples produced in Qinghai, Tibet, and Sichuan provinces were analyzed and compared to investigate the relationship among 26 different producing areas.Materials and Methods: Proteins from 26 different producing areas were extracted by Tris-HCl buffer with Triton X-100, and separated using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and two-dimensional electrophoresis (2-DE).Results: The SDS-PAGE results indicated that the number of protein bands and optical density curves of proteins in 26 CS samples was a bit different. However, the 2-DE results showed that the numbers and abundance of protein spots in protein profiles of 26 samples were obviously different and showed certain association with producing areas.Conclusions: Based on the expression values of matched protein spots, 26 batches of CS samples can be divided into two main categories (Tibet and Qinghai) by hierarchical cluster analysis.

Keywords: Hierarchical cluster analysis; Natural Cordyceps sinensis; Sodium dodecyl sulfate-polyacrylamide gel electrophoresis; Soluble protein; Two-dimensional electrophoresis

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