Review · Volume 5, Issue 10 · pp. 155–158

High-performance liquid chromatography analysis of plant saponins: An update 2005-2010

Jagmohan S. Negi^1,2, Pramod Singh¹, Geeta Joshi Nee Pant¹, M. S. M. Rawat¹

¹Department of Chemistry, HNB Garhwal University, Srinagar (Garhwal).
²Herbal Research and Development Institute, Gopeshwar (Chamoli), Uttarakhand, INDIA.

Abstract

Saponins are widely distributed in plant kingdom. In view of their wide range of biological activities and occurrence as complex mixtures, saponins have been purified and separated by high-performance liquid chromatography using reverse-phase columns at lower wavelength. Mostly, saponins are not detected by ultraviolet detector due to lack of chromophores. Electrospray ionization mass spectrometry, diode array detector, evaporative light scattering detection, and charged aerosols have been used for overcoming the detection problem of saponins.

Keywords: Charged aerosol; diode array detector; evaporative light scattering detection; ESI; high-performance liquid chromatography; mass spectrometry; mobile phase; potodiode array detectors; saponins; sapogenins; ultraviolet

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