Article · Volume 14, Issue 2 · pp. 369–374

Eco-Friendly Enzyme Production: Utilizing Invasive Wedelia trilobata L. for Cellulase Synthesis with Aspergillus niger

Rejitha Lalitha Rajappan¹, Chacko Suju Skaria¹

¹Department of Botany, Mar Ivanios College, Thiruvananthapuram, Kerala, INDIA.

Abstract

Aim: This study explores Wedelia trilobata as a substrate for cellulase production by Aspergillus niger via Solid-State Fermentation and evaluates antibacterial activity of its extracts.Materials and Methods: SSF was performed and cellulase quantified using DNS; antibacterial activity assessed by well diffusion.Results: Maximum enzyme activity observed at 120 hr; protein peaked at 831 µg/mL. Antibacterial assays showed highest inhibition 15 mm against E. coli.Conclusion: Wedelia trilobata is a viable substrate for cellulase production and a source of antibacterial compounds, offering eco-friendly applications.

Keywords: Wedelia trilobata; Cellulase; Solid-State Fermentation; Antibacterial

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