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Asian Journal of Biological and Life sciencesNOW IN
Article

Eco-Friendly Enzyme Production: Utilizing Invasive Wedelia trilobata L. for Cellulase Synthesis with Aspergillus niger

Rejitha Lalitha Rajappan1★, Chacko Suju Skaria1★ Corresponding author

  1. 1Department of Botany, Mar Ivanios College, Thiruvananthapuram, Kerala, INDIA.

CORRESPONDENCE

Rejitha Lalitha Rajappan

Assistant Professor, Department of Botany, Mar Ivanios College, Thiruvananthapuram, Kerala, INDIA.

rejitha.lr@mic.ac.in

Received: 06-02-2025; Revised: 24-04-2025; Accepted: 20-06-2025.

Volume 14, Issue 2 · pp. 369–374 · PUBLISHED May-Aug 2025 · DOI: 10.5530/ajbls.20251453

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ABSTRACT

Aim: This study explores Wedelia trilobata as a substrate for cellulase production by Aspergillus niger via Solid-State Fermentation and evaluates antibacterial activity of its extracts. Materials and Methods: SSF was performed and cellulase quantified using DNS; antibacterial activity assessed by well diffusion. Results: Maximum enzyme activity observed at 120 hr; protein peaked at 831 µg/mL. Antibacterial assays showed highest inhibition 15 mm against E. coli. Conclusion: Wedelia trilobata is a viable substrate for cellulase production and a source of antibacterial compounds, offering eco-friendly applications.

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    Rajappan, R. L., & Skaria, C. S. (2025). Eco-Friendly Enzyme Production: Utilizing Invasive Wedelia trilobata L. for Cellulase Synthesis with Aspergillus niger. Asian Journal of Biological and Life sciences, 14(2), 369–374. https://doi.org/10.5530/ajbls.20251453