Sphaeranthus indicus: A Promising Antimicrobial Agent

Abstract

Objectives: The aim of the present study was to determine the antimicrobial property of the solvent extracts of flowers and aerial parts of S. indicus.Methods: The flowers and the aerial parts of Sphaeranthus indicuswere extracted with n-hexane, benzene, chloroform, ethyl acetate and acetone respectively. The extracts were analyzed for the antimicrobial effect by disc diffusion method at concentrations of 5, 2.5 and 1.25 mg/disc. The Minimum Inhibitory Concentration (MIC) was tested using broth micro dilution method at concentrations ranging from 5 to 0.039 mg/ml.Results: There was a significant antibacterial and antifungal activity in hexane extract of flower and aerial parts. The MIC was seen at 0.15 mg/ml against Staphylococcus aureus and the highest MIC (5 mg/ml) was noted for S. epidermidis. The n-hexane extracts of flower and aerial parts showed MIC as 0.15 and 1.25 mg/ml respectively against Candida albicans.Conclusion:Concluding it can be said that the S. indicusflower n-hexane extract showed promising antimicrobial agent.

Keywords: Sphaeranthus indicus; Antibacterial activity; Minimum inhibitory concentration; Ethyl acetate; Acetone; n-hexane