Article · Volume 33, Issue 1 · pp. 18–23
Enhanced production of Andrographolide from cell cultures of Andrographis paniculata by the elicitaion with Arachidonic acid
Yamjala Ganeshkumar1, Perala Kavitha1, Ajmera Ramarao1, Ciddi Veeresham*1
- 1Department of Pharmacognosy, University College of Pharmaceutical Sciences, Kakatiya University, Warangal, Telangana, India-506009.
Abstract
Background: Andrographis paniculata (Burm. F.) Wall ex. Nees, belonging to the family Acanthaceae produces a group of diterpene lactones, one of which is the pharmaceutically important andrographolide. Hypothesis:It is known to possess various important biological properties like hepatoptroective, anticancer, anti-HIV, anti inflammatory, etc.Method: The production of andrographolide in the cell suspension cultures of Andrographis paniculata by elicitation was investigated.Results: Arachidonic acid enhanced the andrographolide content in the suspension cultures of Andrographis paniculata. The maximum andrographolide production was found to 109.6 0.62% w/w over the control cell cultures 26.40.79% w/w with the addition of arachidonic acid (100 M) on day-7.Conclusion: This study could pave the way for establishing a continuous and higher production of andrographolide, which has an enormous pharmacological importance
Keywords: Andrographis paniculata; Arachidonic acid; suspension cultures; and HPLC
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