Article · Volume 47, Issue 4 · pp. 73–78
Pre-Clinical Evolutionary Study of Alpha-Pinene in L-Arginine Induced Acute Pancreatitis in Rat.
Sandeep Biradar1, Veeresh B1
- 1*Jagdishprasad Jhabarmal Tibrewala University, Vidyanagari, Churu Jhunjhunu Road, Chudela, District-Jhunjhunu, Rajasthan-333001 Department of Pharmacology, G. Pulla Reddy College of Pharmacy, Mehdipatnam, Hyderabad 500 028, India.
Abstract
Objective: Screening of alpha-pinene on L-arginine induced acute pancreatitis model.Material and Methods: In this model the acute pancreatitis was determined at 24h by determination of pancreatic wet weight/body weight ratio, nitrate/nitrite levels, lipid peroxidation (thiobarbituric acid reactive substances (TBARS)], proinflammatory cytokines [tumor necrosis factor (TNF)-α, C-reactive proteins (CRP) and interleukin (IL)], pancreatic myeloperoxidase (MPO) activity and serum levels of amylase and lipase.Results: Administration of L-arginine had induced pancreatitis and this was characterized by pulmonary complication including altered pancreatic wet weight/body weight ratio, nitrate/nitrite levels, proinflammatory cytokines, TBARS, MPO and serum levels of amylase and lipase. Treatment with alpha-pinene (100 and 200mg/kg) dose significantly attenuated the L-arginine-induced pancreatitis and related complication.Conclusion: The histological findings proved the amelioration of pancreatic injury by alphapinene and biochemical parameters proved anti-inflammatory and antioxidant property of alpha-pinene.
Keywords: Acute pancreatitis; Anti-inflammatory; Cytokines; L-arginine; alpha-pinene
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