Article · Volume 49, Issue 4 · pp. 353–361
Quantification of Flavonoids and Nucleoside by UPLC-MS in Indian Cordyceps sinensis and its Invitro Cultures
Masood Shah Khan1,2, Washim Khan1, Manimaran Manickam3, Raj kumar Tulsawani3, Kshipra shipra Misra3, Puran Singh Negi4, Sayeed Ahmad1
- 1Bioactive Natural Product Laboratory, Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Hamdard University, Hamdard Nagar, New Delhi, INDIA.
- 2Department of Pharmacy, Faculty of Science, Mohammad Ali Jauhar University, Rampur, UP.
- 3Defence Institute of Physiology and Allied Sciences, Delhi, INDIA.
- 4Defence Institute of Bio-Energy Research, Haldwani, INDIA.
Abstract
Background: Cordyceps sinensis, a comestible mushroom growing in Himalayan regions, is extensively familar in traditional system of medicine. The paper presents the results of quantification of some of the flavonoids and nucleosides (kaempferol, isorhamnetin and cordycepin) in the Indian variety of Cordyceps sinensis. Since, the plant is known for its high therapeutic potential and not easily available, hence it was also tissue cultured in the laboratory as an alternative source of medicine.Methods: Ultra Performance Liquid Chromatography (UPLC) along with Q-TOF–MS detector was used for the characterization and quantification of active components in the aqueous extracts of both the samples of Cordyceps sinensis (natural as well as in vitro cultured).Results: Results indicate that both the extracts have almost similar concentration of the kaempferol, isorhamnetin and cordycepin which supports use of in vitro biomass as an alternative source of medicine.
Keywords: Cordycepin; Cordyceps sinensis; Isorhamnetin; Kaempferol; UPLC-MS/MS
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