Article · Volume 51, Issue 3S2 · pp. s508–s512

Effects of Methyl Jasmonate and Caffeic Acid Applications on Secondary Metabolite Production in Madder (Rubia tinctorum) Root Cultures

Pınar Özdamar Biçer¹, Tunhan Demirci¹, Özlem Aras Aşcı², Nilgün Göktürk Baydar¹

¹Süleyman Demirel University, Agricultural Faculty, Department of Agricultural Biotechnology, 32260 Isparta, TURKEY.

Abstract

This study was carried out to determine the effects of methyl jasmonate (MeJA) and caffeic acid (CA) applications on the root growth index and contents of total anthraquinones (AQs), alizarin, purpurin, total phenolic (TP) and individual phenolic compounds in madder (Rubia tinctorum) roots grown in vitro conditions. For this aim, in vitro adventitious roots derived from internode parts were cultured in Murashige and Skoog media containing two different concentrations of MeJA (10 and 100 μM) and CA (1 and 2 mM) for 7 days. Based on the results, MeJA decreased the root growth index compared to control while CA had a positive influence on the root growth of madder. It may be concluded that 2 mM CA+100 μM MeJA combination was the most suitable application providing the highest AQs and phenolics.

Keywords: Rubia Tinctorum; Root Culture; Caffeic Acid; Methyl Jasmonate; secondary; metabolite; madder

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