Article · Volume 51, Issue 4 · pp. 684–691
Response Surface Methodology Optimization of Thebaine Biotransformation into Codeine and Morphine Using Bacillus sp. FAR
Nazanin Ataee1, Jamshid Fooladi2, Mohammad Hasan Namaei3, Fateme Mirzajani4
- 1Department of Microbiology, Faculty of Biological Science, Alzahra University, Vanak, Tehran, IRAN.
- 2Department of Biotechnology, Faculty of Biological Science, Alzahra University, Vanak, Tehran, IRAN.
- 3Birjand Infectious Diseases Research Center, Birjand University of Medical Sciences, Ghafary Ave., Birjand, IRAN.
- 4Departmrnt of Nanobiotechnology, Protein Research Center (PRC), Shahid Beheshti University, G.C., Tehran, IRAN.
Abstract
Background: Codeine and morphine are important pharmaceuticals having analgesic characteristics.Aim: The current study used response surface methodology to optimize the biotransformation of thebaine into codeine and morphine using Bacillus sp. FAR.Methods: A central composite design was used to determine the optimal concentrations of buffer and thebaine, pH, time, temperature, and biomass. Improvement in transformation was monitored using HPLC-DAD.Results: The optimized conditions employed were pH 7.7 at 36°C for 30 h using a thebaine concentration of 80 μg/mL, 45 mM tris of buffer and 2.9% w/v biomass. The optimal conditions for codeine and morphine production were 2.995 and 1.113 μg, respectively.Conclusion: Optimization demonstrated that pH and biomass had the strongest effects on biotransformation.
Keywords: Biotransformation; CCD; Codeine; Morphine; Thebaine; Bacillus
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