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IJPER · Lens · Antioxidant Properties and Protective Effect of Turkish Propolis on t-BHP-Induced Oxidative Stress in Foreskin Fibroblast Cells

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Article · Volume 52, Issue 1 · pp. 94–100

Antioxidant Properties and Protective Effect of Turkish Propolis on t-BHP-Induced Oxidative Stress in Foreskin Fibroblast Cells

Sema Misir1,2, Yuksel Aliyazicioglu3, Selim Demir4, Ibrahim Turan5,6, Serap Ozer Yaman2, Orhan Deger3

  1. 1Department of Biochemistry, Faculty of Pharmacy, Cumhuriyet University, Sivas, TURKIYE.
  2. 2Department of Medical Biochemistry, Institute of Health Sciences, Karadeniz Technical University, Trabzon, TURKIYE.
  3. 3Department of Medical Biochemistry, Faculty of Medicine, Karadeniz Technical University, Trabzon, TURKIYE.
  4. 4Department of Nutrition and Dietetics, Faculty of Health Sciences, Karadeniz Technical University, Trabzon, TURKIYE.
  5. 5Department of Genetic and Bioengineering, Faculty of Engineering and Natural Sciences, Gumushane University, Gumushane, TURKIYE.
  6. 6Medicinal Plants, Traditional Medicine Practice and Research Center, Gumushane University, Gumushane, TURKIYE.

Abstract

Objective: Propolis is an important bee product, rich in polyphenolic compounds. It has antitumoral, antioxidant, antimutagenic, and other useful activities. Biological activities of propolis are generally attributed to its substance of polyphenolic compounds. The aim of this study was to investigate the in vitro antioxidant properties and reduction amount of intracellular reactive oxygen species (ROS) in human normal foreskin fibroblast cells by Turkish propolis ethanolic extract (EEP).Method: Total phenolic and flavonoid contents, reducing power, radical scavenging capacity, and iron chelating activity of EEP were determined using spectrophotometric methods. Intracellular ROS levels were determined by spectrofluorometric analysis with CM-H2DCFDA.Results: Our results indicated that antioxidant activity was correlated with the higher total phenolic and flavonoid contents of EEP. The generated ROS levels were significantly decreased by EEP compared to only t-BHP group.Conclusion: Ethanolic propolis extract may be evaluated as a potency antioxidant resource and/or a novel natural agent in food and drug industries.

Keywords: Antioxidant activity; CM-H2DCFDA; Fibroblast cells; Polyphenols; Propolis

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