Article · Volume 54, Issue 2 · pp. 465–470
HPLC Method for Determination of the Chemical Stability of Antimicrobial Peptide α-Defensin 2
Vania Maslarska1, Meri Amerikova2, Ivanka Pencheva2, Stanislav Bozhanov1
- 1Department of Chemistry, Faculty of Pharmacy, Medical University-Sofia, 2 Dunav St., 1000 Sofia, BULGARIA.
- 2Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Medical University-Sofia, 2 Dunav St., 1000 Sofia, BULGARIA.
Abstract
Aim: The aim of this research study was to develop a simple, rapid, precise, accurate and economical RP-HPLC method, with a simple mobile phase for the identification and determination of the antimicrobial peptide α-defensin-2.Materials and Methods: Separation was carried out at 25°C, using column Luna 5U (C18, 250x4.6, 5 μm) with mobile phase consisting of acetonitrile: water (40: 60 v/v). The detector was set at 210 nm. The flow rate was 1.2 ml/min and injection volume was 20 μl.Results: The developed method was validated as per ICH guidelines and the kinetic behavior of α-defensin 2 was studied in respect of different pH.Conclusion: The obtained data can serve for creating an in vivo monitoring program and quality control for different studies and preparations.
Keywords: Antimicrobial peptides; α-defensin 2; HPLC; Chemical stability; Analysis
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