Article · Volume 54, Issue 2S · pp. s295–s300

In-vitro Cytotoxicity and in silico Molecular Docking of Alkaloids from Tiliacora acuminata

Jeswiny Rodrigues¹, Kirankumar Hullatti¹, Sunil Jalalpure¹, Pukar Khanal²

¹Department of Pharmacognosy, KLE College of Pharmacy, Belagavi, KLE Academy of Higher Education and Research (KAHER), Belagavi, Karnataka, INDIA.
²Department of Pharmacology and Toxicology, KLE College of Pharmacy, Belagavi, KLE Academy of Higher Education and Research (KAHER), Belagavi, Karnataka, INDIA.

Abstract

Background: The present study aimed to identify the cytotoxicity of Tiliacora acuminata extract/fraction(s) plant via bioactivity guided approach and predict the binding affinity with Topoisomerase II.Materials and Methods: Extract and fractions were screened using brine shrimp lethality bioassay and the potent fraction was further evaluated for its in vitro cytotoxicity using five different cell lines i.e. HT-29, HepG2, MCF-7 and A-549. The binding affinity of individual phytoconstituent from the potent fraction with Topoisomerase II was further predicted using autodock4.Results: The fraction containing alkaloids showed the highest cytotoxicity against the HT-29 cell line. Molecular docking study identified coclaurine as a potent alkaloid phytoconstituent to bind with topoisomerase II.Conclusion: The study revealed the potential cytotoxic alkaloids from T. acuminata which may find use in the development of cytotoxic or chemopreventive agents.

Keywords: Alkaloids; Brine shrimp; Docking; Menispermaceae; MTT; Topoisomerase II

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