jrnls.org logo
Submit

BROWSE ALL JOURNALS

Type 2 letters to search all 19 journals

SEE ALL 19 JOURNALS
Indian Journal of Pharmaceutical Education and ResearchNOW IN
Back

IJPER · Lens · Evaluation of iceA1 Gene Expression of Helicobacter pylori Risk Factor of Gastric Cancer in Transgenic Brinjal

PDF

Article · Volume 55, Issue 4 · pp. 1197–1206

Evaluation of iceA1 Gene Expression of Helicobacter pylori Risk Factor of Gastric Cancer in Transgenic Brinjal

Mohammad Javad Mehran1, Basaralu Yadurappa Sathish Kumar1,2, Nanjundappa Haraprasad3, Rambod Barzigar1, Bashasab Fakrudin4, Sayan Paul5,6

  1. 1JSS Research Foundation, SJCE Technical Campus, Mysore, Karnataka, INDIA.
  2. 2Department of Biotechnology, JSS College, Ooty Road, Mysore, Karnataka, INDIA.
  3. 3JSS Science and Technology University, SJCE, Technical Campus, Mysore, Karnataka, INDIA.
  4. 4Department of Biotechnology and Crop Improvement, College of Horticulture, University of Horticulture Sciences Campus, GKVK Post, Bengaluru, Karnataka, INDIA.
  5. 5Department of Biotechnology, Manonmaniam Sundaranar University, Tirunelveli, Tamil Nadu, INDIA.
  6. 6Centre for Cardiovascular Biology and Disease, Institute for Stem Cell Science and Regenerative Medicine (inStem), Bangalore, Karnataka, INDIA.

Abstract

Background: The advancement of plant biotechnology improved crop production by revolutionizing plant science. Humans were commonly infected by Helicobacter pylori, and it was closely linked to stomach ulcers and cancer. In addition to traditional vaccines for H. pylori, transgenic plants have also been produced to produce its antigens as well as edible and non-edible parts that can produce an immune response after consumption. The protein present in H. pylori associated with virulence (iceA1) is believed to predispose to stomach cancer.Objectives: The current study was aimed to evaluate the iceA1 gene expression within brinjal plant to produce novel transgenic lines LC420461-B7, -B10, -B15, -B22 and -B27.Materials and Methods: In the present study, amplified iceA1 gene from H. pylori strain 26695 was transformed into callus of brinjal (from leaf explants) through Agrobacterium tumefacians (EHA105). pBI121 vector was used in constructing the plant expression vector, and the transgenics generated were further evaluated by quantitative Real-Time PCR and western blot analysis.Results: Out of the 46 plants obtained five of them were found to be positive for the iceA1 expression. Both real time and western blot confirmed of the presence of expressed gene iceA1 within the plant sample leaves. By studying transgenic brinjal, the study may result in an H. pylori vaccine candidate. As well, the data can be used by researchers to get valid scientific information.

Keywords: Helicobacter pylori; Transgenic brinjal; iceA1; Agrobacterium; Callus induction; Real-time PCR

The full text of this article is available as a PDF. Open the PDF.