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IJPER · Lens · Formulation, Characterization, and Evaluation of Doxorubicin-loaded Cubosome as a Cytotoxic Potentiator against HCT-116 Colorectal Cancer Cells

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Article · Volume 56, Issue 3 · pp. 723–731

Formulation, Characterization, and Evaluation of Doxorubicin-loaded Cubosome as a Cytotoxic Potentiator against HCT-116 Colorectal Cancer Cells

Yosif Almoshari1, Mohammad Intakhab Alam1, Mohammed Ali Bakkari1, Ahmad Salawi1, Meshal Alshamrani1, Fahad Y Sabei1, Awaji Y Safhi1, Jawaher Abdullah Alamoudi2, Ibrahim M Ibrahim3

  1. 1Department of Pharmaceutics, College of Pharmacy, Jazan University, Jazan, SAUDI ARABIA.
  2. 2Department of Pharmaceutical Sciences, College of Pharmacy, Princess Nourah bint Abdulrahman University, Riyadh, SAUDI ARABIA.
  3. 3Department of Pharmacology, Faculty of Medicine, King Abdulaziz University, Jeddah, SAUDI ARABIA.

Abstract

Background: Colorectal cancer (CRC) has grown to be the world’s fourth-biggest cause of cancer-related mortality. It is critical to identify more effective treatment options for it.Objectives: Doxorubicin a potent antineoplastic agent is widely used but has severe adverse effects. Therefore, our objective is to use cubosome as an efficient drug delivery system to reduce the off-target effects and increase the cytotoxic effects in CRC cells.Materials and Methods: Pluronic F127-Based Cubosomes were prepared by low energy stirring method and loaded with doxorubicin. Physicochemical characteristics were studied using X-ray diffraction, dynamic light scattering, microscopic techniques, FTIR UV spectrophotometer, and entrapment efficiency. In vitro activity of doxorubicin-loaded cubosomes was studied on HCT-116 cells.Results: The prepared cubosomes have the required nano-size and the FTIR results showed the presence of both doxorubicin and Pluronic F-127. The entrapment efficiency of doxorubicin was high too. DOX-CBs have a better cytotoxic effect and induced ROS and reduced NO levels in HCT-116 cells.Conclusion: The obtained results show that doxorubicin-loaded cubosomes can be used to increase the cytotoxic effect in CRC cells and can be used with other chemotherapeutic agents.

Keywords: Doxorubicin; HCT-116 cells; Pluronic F127; in vitro activity; Cubosomes; Entrapment efficiency

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