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IJPER · Lens · Investigation of in vitro and in vivo Pursuits of Laurus nobilis Extract on Acetic Acid-induced Ulcerative Colitis in Wistar Rats

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Article · Volume 57, Issue 3S · pp. s644–s651

Investigation of in vitro and in vivo Pursuits of Laurus nobilis Extract on Acetic Acid-induced Ulcerative Colitis in Wistar Rats

Suliman Abdullah Al-Omran1, Virdala Chinnikrishnaiah2, Vakkalagadda Siva Ganesh3, Prakash Goudanavar3, Nimbagal Raghavendra Naveen3, Santosh Fattepur4, Girish Meravanige Basavarajappa5, Predeepkumar Narayanappa Shiroorkar5, Mallikarjun Telsang6, Sreeharsha Nagaraja1,7

  1. 1Department of Pharmaceutical Sciences, College of Clinical Pharmacy, King Faisal University, Al-ahsa, SAUDI ARABIA.
  2. 2Department of Pharmacology, Annamacharya College of Pharmacy, New Boyanapalli, Rajampet, Andhra Pradesh, INDIA.
  3. 3Department of Pharmaceutics and Pharmacology, Sri Adichunchanagiri College of Pharmacy, Adichunchanagiri University, B.G. Nagar, Karnataka, INDIA.
  4. 4Department of Pharmacology, School of Pharmacy, Management and Science University, Selangor, MALAYSIA.
  5. 5Department of Biomedical Sciences, College of Medicine, King Faisal University, Al-Ahsa, SAUDI ARABIA.
  6. 6Department of Surgery, College of Medicine, King Faisal University, Al-Ahsa, SAUDI ARABIA.
  7. 7Department of Pharmaceutics, Vidya Siri College of Pharmacy, Off Sarjapura Road, Bangalore, Karnataka, INDIA.

Abstract

Background: The ethanolic extract of Laurus nobilis possesses an anti-inflammatory effect, which will protect against acetic acid-induced Ulcerative colitis by inhibiting endogenous free radicals.Objectives: The work has been undertaken to explore the efficacy and safety of ethanolic leaf extract of Laurus nobilis in treating acetic acid-induced Ulcerative colitis by estimating the biochemical, physical parameters, and histopathological studies.Materials and Methods: The plant powder of L. nobilis was extracted using 70% ethanol, and a phytochemical investigation was performed. Male and Female Wistar albino rats were divided into six groups and pretreated with saline, saline followed by ulcer induction, two doses of extracts (100mg/kg and 200mg/kg b.w), and sulfasalazine (one day) followed by ulcer induction and one low dose extract treatment without inducing ulcer.Results: The phytochemical investigation of the extract confirms the presence of flavonoids. The biochemical estimations showed increased SOD, CAT, and GSH and decreased levels of TBARS in extract-treated groups compared to the acetic acid-induced group. The physical parameters like Crypt abscesses, gross mucosal inflammation, wet weight of the colon, diarrhea, and activity score have also shown better results in extract-treated groups showing a protective effect. Histopathological results further confirmed the therapeutic effect of the extract.Conclusion: The protective effect of ethanolic leaf extract of Laurus nobilis has been proved. The therapeutic effect was mainly due to flavonoids, and the low-dose extract was more potent than the high-dose extract.

Keywords: Laurus nobilis; Ulcerative Colitis; Anti-oxidant; Histological Studies; Ethanolic Extract

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