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Indian Journal of Pharmaceutical Education and ResearchNOW IN
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IJPER · Lens · Palliative Action of Woodfordia fruticosa Leaves Containing Woodfruticosin on Biological Redox Imbalance and Anemia in CFA-Induced Arthritic Rats

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Article · Volume 57, Issue 4 · pp. 1087–1097

Palliative Action of Woodfordia fruticosa Leaves Containing Woodfruticosin on Biological Redox Imbalance and Anemia in CFA-Induced Arthritic Rats

Ashish Singhai1,2, Yusra Ahmad2, Umesh Kumar Patil3

  1. 1College of Pharmacy, Teerthanker Mahaveer University, Uttar Pradesh, INDIA.
  2. 2Department of Pharmacy, VMSB Uttarakhand Technical University, Dehradun, Uttarakhand, INDIA.
  3. 3Department of Pharmaceutical Sciences, Dr. Harisingh Gour Vishwavidyalaya (A Central University), Sagar, Madhya Pradesh, INDIA.

Abstract

Background: Rheumatoid Arthritis (RA) is a chronic disorder affecting musculoskeletal and autoimmune system with inflammatory characteristics. Anemia is most prevailing, among other extra-articular complications in RA. These complications are analogous to exorbitant inflammation and biological redox imbalance which ultimately affects the quality life and life expectancy of RA patients.Objectives: The study was planned to investigate the palliative action of Woodfordia fruticosa (WF) leaf extracts and isolation from active fraction, on articular/ extra-articular complications in rats with Complete Freund’s Adjuvant (CFA) induced arthritis.Materials and Methods: The action of Petroleum ether (WFP) and Ethanolic (WFE) extracts of WF was assessed in CFA induced adult Lewis rats (180-200gm) arthritic rats. In prophylactic treatment they received WF extracts 100mg/kg p.o. for 28 successive days and therapeutic treatment from the 14th day of CFA injection. Active extract was then fractionated by solvent-solvent extraction and chromatographic techniques. The isolated compound was characterized by TLC and various spectroscopic studies.Results: WFP significantly alleviated the severity of arthritis in animals than WFE in 28 days. Remarkable reduction (p < 0.001) in various complications was observed including paw volume (85.42%), loss in body weight, erythrocyte sedimentation rate, anemia, locomotor activity, and lipid peroxidation of hepatic/ synovial tissues and increment in SOD (U/mg) activities. A compound was isolated and characterized by UV, TLC, FTIR, LCMS and NMR spectroscopic techniques.Conclusion: WFP at 100mg/kg was advantageous in the superintendence of RA intricacies which was also confirmed by the histological and radiological studies. And a tannin compound Woodfruticosin was isolated.

Keywords: Rheumatoid arthritis; Chromatography; Isolation; Spectroscopy; Woodfruticosin

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