Article · Volume 57, Issue 4 · pp. 1226–1231

Interaction Studies of Moxifloxacin with Anti-diabetic Drugs and Application of RP-HPLC in Analysis

Sania Bashir¹, Fatima Qamar¹, Safila Naveed¹, Syeda Zainab², Halima Sadia¹, Aisha Sana¹, Wajeeha Muzafar³

¹Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Jinnah University for Women, Karachi, PAKISTAN.
²Department of Pharmaceutics, Jinnah University for Women, Karachi, PAKISTAN.
³Department of Chemistry, H.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi, PAKISTAN.

Abstract

Aim: Simple RP-HPLC method was developed and validated for the simultaneous estimation of Glimepiride, Glibenclamide and Moxifloxacin in pharmaceutical preparation.Materials and Methods: Mediterranean C18 column with dimensions of 150mm×4.6mm was used with HPLC Shimadzu LC-20 AT model, Version 1:62 is used for processing of chromatograms at room temperature (25± 2°C). 1 mL per minute was the flow rate and detector were set at 254nm with mobile Phase consisting of 85:10:5 v/v methanol: water: acetonitrile.Results: The developed method proved to be accurate, simple, effective precise and reproducible. One way ANOVA was also applied for further confirmation of the results.Conclusion: The method is applicable for the routine analysis of drugs in API and in formulation and for studying in vitro interaction studies.

Keywords: Moxifloxacin; Glimepiride; Glibenclamide; in vitro interaction studies

The full text of this article is available as a PDF. Open the PDF.