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Indian Journal of Pharmaceutical Education and ResearchNOW IN
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IJPER · Lens · Quantification of Miconazole Nitrate and Eugenol in Formulated Emulgel by Planar Chromatography along its Stability Studies

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Article · Volume 59, Issue 3S · pp. s1134–s1140

Quantification of Miconazole Nitrate and Eugenol in Formulated Emulgel by Planar Chromatography along its Stability Studies

Aarti Sachin Zanwar1, Dhanya B Sen1, Pruthvi Hiteshkumar Vyas1, Sushil Sureshrao Burle2, Dipti Bipinchandra Ruikar3, Ashim Kumar Sen1

  1. 1Department of Pharmacy, Sumandeep Vidyapeeth (Deemed to be University), Piparia, Waghodia, Vadodara, Gujarat, INDIA.
  2. 2Department of Pharmaceutical Chemistry, Smt. Kishoritai Bhoyar College of Pharmacy, Kamptee, Nagpur, Maharashtra, INDIA.
  3. 3Department of Pharmaceutical Chemistry, P. R. Pote Patil College of Pharmacy, Kathora Naka, Amravati, Maharashtra, INDIA.

Abstract

Introduction: Fungal infections are rapidly increasing and combination therapy using Miconazole Nitrate (MCZ) and Eugenol (EGN), may offer a promising approach for developing new antifungal medications.Objectives: The purpose of the demonstrated work was to develop and validate a stability-indicating HPTLC method for concurrent measurement of MCZ and EGN in formulated emulgel.Materials and Methods: The separation technique of the HPTLC method was aided by pre-coated silica gel 60F254 on aluminium and toluene: ethyl acetate: methyl alcohol (18:1:1 v/v/v) as mobile phase. The quantification of MCZ and EGN in formulated emulgels along with their stability studies was conducted.Results: The optimised conditions were used to develop the spotted drugs and showed a linear response from 100-600 ng/band (r2=0.9995) for MCZ at Rf value of 0.33 and 53-318 ng/band (r2=0.9996) for EGN at Rfvalue of 0.70.Conclusion: The proposed validated work is suitable for the quantification of the above-cited drugs under various stress conditions and quality control purpose.

Keywords: Eugenol; Miconazole Nitrate; HPTLC; Emulgel; Topical formulation

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