Influence of Schisandrin A on Serum TNF-α, IL-1β, D-Lactic Acid, Diamine Oxidase and Colonic Tissue Oxidative Stress Markers in a Murine Model of Ulcerative Colitis

Abstract

Introduction: This study was to demonstrate the potential roles and effects of Schisandrin A in the therapy of Ulcerative Colitis (UC).Materials and Methods: 60 mice from the China Experimental Animal Center were purchased and the mice were assigned to Group A (normal control), Group B (UC model), Group C1 (80 mg/kg Schisandrin A), Group C2 (40 mg/kg Schisandrin A), Group C3 (20 mg/kg Schisandrin A) and Group D (sulfasalazine), each comprising 10 individuals. An UC model was established and various parameters including colonic mass, colon length, colon weight index, spleen index and disease activity index were compared across groups.Results: The results revealed marked decreases in colonic mass, colon weight index and spleen index in Group B mice versus Group A. Conversely, colon length, disease activity index, TNF-α, IL-1β, DAO, D-LA and colonic tissue Oxidative Stress (OS) markers showed substantial elevation (p<0.05) in Group B. Comparing Group B with Groups C1, C2, C3 and D indicated varying degrees of improvement in colonic mass, colon length, colon weight index, spleen index, disease activity index, colonic tissue TNF-α, IL-1β levels, as well as serum DAO and D-LA levels and colonic tissue OS markers (p<0.05).Conclusion: In summary, Schisandrin A exhibited the ability to alleviate inflammatory reactions, suppress OS responses and improve symptoms and inflammation severity in UC mice.

Keywords: Inflammatory factors; Oxidative stress; Schisandrin A; Ulcerative colitis