Article · Volume 11, Issue 4 · pp. 354–357

Anticancer Activity of Elytraria acaulis L. Extracts on Triple Negative Breast Cancer Cell Line

Suresh Babu Bastipati¹, Chepuri Kalyani¹, Chidepudi Devi Sri Lalitha Naga Tulasi¹, Lavudi Saida¹

¹Centre for Biotechnology, Institute of Science and Technology, Jawaharlal Nehru Technological University, Hyderabad, Telangana, INDIA.

Abstract

Objectives: In the present study, the anticancer potential of the plant extracts of Elytraria acaulis L. (E. acaulis L.) were investigated on human breast cancer cells (MDAMB-231).Methods: Different organic solvent extracts of E. acaulis L. were prepared using methanol, ethanol, ethyl acetate and hexane. The cytotoxicity of plant extracts were evaluated by MTT assay (-(4, 5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) against MDA MB-231cells. The apoptosis associated morphological changes were also observed with the help of propidium iodide staining method.Results: Of all the solvent extracts, methanol extract has shown the highest activity and its IC50 concentration was found to be 80.8±2.08 μg/ml. The anticancer drug Cis-diammineplatinum (II) dichloride was considered as a standard.Conclusion: In further the bioactive compound should be isolated from the methanol crude extract, which is responsible for the cytotoxic effects.

Keywords: E. acaulis L; Anticancer activity; Cytotoxic assay; Propidium iodide; Apoptosis

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