Spectrophotometric Determination of Favipiravir in Bulk and Pharmaceutical Formulation Using Bromothymol Blue Reagent

Abstract

Background: The primary objective of this research was to establish a spectrophotometric technique to quantitatively analyze the concentration of favipiravir in both its pure form and pharmaceutical preparations using bromothymol blue reagent.Materials and Methods: In this method, a yellow-colored chromagen was developed when favipiravir reacted with bromothymol blue reagent. Acetonitrile was selected as a solvent and the colored complex was detected at a wavelength of 475 nm.Results: The validation of the developed method was conducted following the guidelines set by the International Council for Harmonisation (ICH). The results demonstrated a strong linear relationship within the concentration range of 10-50 µg/ mL, exhibiting a correlation coefficient of 0.9995. Moreover, the developed method exhibited excellent accuracy, precision, specificity, and sensitivity Conclusion: For routine analysis purposes, this method can be readily utilized to determine the concentration of favipiravir in both bulk samples and pharmaceutical dosage forms.

Keywords: Favipiravir; Spectrophotometric method; Bromothymol blue; Method development; Validation