Article · Volume 14, Issue 1 · pp. 258–262
Optimization of Lipase Production from Pseudomonas fluorescens
Lekhana Bandi1, Naga Shivani Lakshmisetty1, Keerthana Ankireddy1, Leela Lakshmi Vaishnavi Pulipati1, Asritha Lakshmi Bontu1, Ranganadha Reddy Aluru2, Chandrasekhar Chanda1
- 1Department of Biotechnology, Koneru Lakshmaiah Education Foundation, Vaddeswaram, Andhra Pradesh, INDIA.
- 2Department of Biotechnology, VFSTR, Guntur, Andhra Pradesh, INDIA.
Abstract
Objectives: Lipases are widely used in biodiesel, food, detergent, and pharmaceutical industries. To fulfil the rising demand for these crucial industrial enzymes, an extensive study on lipases from various micro-organisms will be useful in maximising the growing conditions and reducing production costs.Materials and Methods: The current work concentrated on optimising the carbon source, inducer concentration, and growth parameters to produce lipases at a reasonable price from pseudomonas species.Results: Extracellular lipases from Pseudomonas fluorescens were produced and optimised, achieving a concentration of 220 IU/mL of submerged fermentation broth.Conclusion: The results showed that the extracellular lipase production from Pseudomonas fluorescens was enhanced by three folds using submerged fermentation.
Keywords: Lipases; Enzyme production; Pseudomonas fluorescens; Submerged fermentation; Cation Exchange Chromatography
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