Optimization of Lipase Production from Pseudomonas fluorescens
Lekhana Bandi1, Naga Shivani Lakshmisetty1, Keerthana Ankireddy1, Leela Lakshmi Vaishnavi Pulipati1, Asritha Lakshmi Bontu1, Ranganadha Reddy Aluru2, Chandrasekhar Chanda1★★ Corresponding author
- 1Department of Biotechnology, Koneru Lakshmaiah Education Foundation, Vaddeswaram, Andhra Pradesh, INDIA.
- 2Department of Biotechnology, VFSTR, Guntur, Andhra Pradesh, INDIA.
CORRESPONDENCE
Chandrasekhar Chanda
Department of Biotechnology, Koneru Lakshmaiah Education Foundation, Vaddeswaram, Andhra Pradesh, INDIA.
Received: 21-09-2023; Revised: 11-10-2023; Accepted: 04-11-2023.
Volume 14, Issue 1 · pp. 258–262 · PUBLISHED · DOI: 10.5530/ijpi.14.1.32
ABSTRACT
Objectives:Lipases are widely used in biodiesel, food, detergent, and pharmaceutical industries. To fulfil the rising demand for these crucial industrial enzymes, an extensive study on lipases from various micro-organisms will be useful in maximising the growing conditions and reducing production costs. Materials and Methods: The current work concentrated on optimising the carbon source, inducer concentration, and growth parameters to produce lipases at a reasonable price from pseudomonas species. Results: Extracellular lipases from Pseudomonas fluorescens were produced and optimised, achieving a concentration of 220 IU/mL of submerged fermentation broth. Conclusion: The results showed that the extracellular lipase production from Pseudomonas fluorescens was enhanced by three folds using submerged fermentation.
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Bandi, L., Lakshmisetty, N. S., Ankireddy, K., Pulipati, L. L. V., Bontu, A. L., Aluru, R. R., & Chanda, C. (). Optimization of Lipase Production from Pseudomonas fluorescens. International Journal of Pharmaceutical Investigation, 14(1), 258–262. https://doi.org/10.5530/ijpi.14.1.32