jrnls.org logo
Submit

BROWSE ALL JOURNALS

SEE ALL 19 JOURNALS
International Journal of Pharmaceutical InvestigationNOW IN
Article

Protection of Fargesin against Hydrogen Peroxide-induced Cell Death in RAW 264.7 Cells

Amira Nabila Mat Roof1, Richard Johari James1,2, Salfarina Ramli1,2, Boonchoo Sritularak3,5, Pornchai Rojsitthisak3,4, Hasseri Halim1,2★★ Corresponding author

  1. 1Faculty of Pharmacy, Universiti Teknologi MARA (UiTM), Bandar Puncak Alam, Selangor, MALAYSIA.
  2. 2Integrative Pharmacogenomics Institute (iPROMISE), Universiti Teknologi MARA Selangor, Puncak Alam, Selangor, MALAYSIA.
  3. 3Center of Excellence in Natural Products for Ageing and Chronic Diseases, Chulalongkorn University, Bangkok, THAILAND.
  4. 4Department of Food and Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, THAILAND.
  5. 5Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, THAILAND.

CORRESPONDENCE

Hasseri Halim

Faculty of Pharmacy, Universiti Teknologi MARA (UiTM), Bandar Puncak Alam, Selangor, MALAYSIA.

hasseri2945@uitm.edu.my

Received: 28-07-2023; Revised: 25-09-2023; Accepted: 25-10-2023.

Volume 14, Issue 1 · pp. 55–61 · PUBLISHED · DOI: 10.5530/ijpi.14.1.8

View on IJPI original site ↗

ABSTRACT

Background:The imbalance level of Reactive Oxygen Species (ROS) in the body can lead to oxidative stress in cells, resulting in the progression of chronic diseases. Fargesin, a bioactive lignan found in Flos magnoliae, an herb widely used in Chinese herbal medicines, has been identified as a potential compound to mitigate this imbalance. Materials and Methods:Hydrogen peroxide (H2 O2 ), one of the major ROS, is commonly employed as an experimental model to induce oxidative stress. This study aimed to investigate the protective effect of fargesin against cell death induced by hydrogen peroxide in RAW 264.7 macrophage cells. RAW 264.7 macrophage cells were pre-treated with various concentrations of fargesin before being exposed to toxic dose (1 mM or 2 mM) of hydrogen peroxide. The viability of the cells was assessed using the MTT assay, and the morphological changes were described. Results and Discussion: The MTT assay demonstrated that non-toxic doses of fargesin (1.56 µM, 3.13 µM, 6.25 µM, 12.5 µM, and 25 µM) significantly reduced H2 O2 -induced cytotoxicity in the treated cells compared to the control group. Conclusion:This research is expected to provide a basis for future studies in the development of a new antioxidant agent for the treatment and prevention of diseases associated with oxidative stress.

KEYWORDS

Open in new tab

REFERENCES

As published

Showing references and in-text citations exactly as published.

    Cite this article

    SELECT FORMAT

    Roof, A. N. M., James, R. J., Ramli, S., Sritularak, B., Rojsitthisak, P., & Halim, H. (). Protection of Fargesin against Hydrogen Peroxide-induced Cell Death in RAW 264.7 Cells. International Journal of Pharmaceutical Investigation, 14(1), 55–61. https://doi.org/10.5530/ijpi.14.1.8